Biomarkers

The Biomarkers Service is dedicated to the identification and characterization of biological markers (biomarkers) correlating with the activity of solid tumors in the endocrine field, which can be expanded to other areas of interest. The research group boasts consolidated experience in the study of genetic markers, cellular markers of response to pharmacological therapies, and the dosage of biologically active molecules. The scientific activity is evidenced by numerous publications in prestigious international journals.

The service is characterized by the use of innovative technologies that make it competitive, particularly AlphaScreen and the various applications of Real Time PCR (RT-PCR).

AlphaScreen is ideal for high-sensitivity screening (in the attomolar range) of the effects of bioactive molecules in terms of activity and biomolecular interactions, utilizing a time-resolved chemiluminescent signal.

RT-PCR, in its TaqMan, Allelic Discrimination, and High Resolution Melting (HRM) variants, allows real-time measurement of the initial concentration of target sequences and the analysis of DNA sequence variations (SNPs, insertions, deletions).

The service offers support for:

• In vitro assays with bioactive molecules on immortalized cell lines (murine, rat, human, canine) and on primary cultures derived from human tissue explants (e.g., adenomas, carcinomas).

• Genotype-phenotype correlation studies.

• Development of customized tests based on the user's needs.

Specifically, it is possible to perform:

• Evaluation of cellular parameters: Viability (MTT, ATP), Apoptosis (caspase 3/7), Hormonal/Peptide secretion (ELISA).

• Analysis of signal transduction pathway activation using AlphaScreen and luciferase activity assays.

• Gene expression analysis and genotyping (TaqMan RT-PCR, Allelic Discrimination).

• Screening for DNA sequence variations (HRM).

• Multi-modality plate reading: Fluorescence intensity, luminescence, absorbance, AlphaScreen.